Dr. Vikas Vasant Patil has completed his PhD at the age of 32 years from North Maharashtra University, Jalgaon Maharashtra, India in the faculty of Medicine and Pharmacy. He is working as a Professor and PG Guide in Affiliated to North Maharashtra University. He has published more than 28 papers in reputed journals and has been serving as an editorial board member and reviewers of reputed Journals. He has guided 25 PG (M.Pharm) students. He is life Member of APTI and INPHARM Association.
In the present study attempt has made to separate terpens from crude drugs (Mangifera Indica Linn). Determination of chemical constituent in percentage by GC and GCMS analysis was done, higher content of α-terpineol (3.26%),β-selinene (3%) found in Totapuri variety other constituents also estimated. Crude drug Vitiveria Zizanoides (Linn) collected from different district region of Maharashtra vetiver oil separated by distillation method. The physical parameters of oils are determined according to ISI specifications. Percentages of constituents are estimated observed that Bharatpur variety having high % of free alcohol as vetiverol (78.10%) The odorous volatile principles of plant and animal sources are known as volatile oils, as they evaporate when exposed to air at ordinary temperature, they are also called as Ethereal oils. They represent essence or active constituents of plant, hence they are also known as Essential oils chemically they are derived from terpenes and their oxygenated compounds and they are made up of isoprene units (C5H8)
Gae-ddong-ssuk, Artemisia Annua L. is an annual herb belonging to Asteraceae family which is grown popularly in Asia including Korean and China. The herb is well known for the effects of fever, hemostasis, and antibiotics. When the mouse macrophage cell line, Raw264.7 was stimulated with LPS (lipopolysaccharide), the cells tremendously secreted pro-inflammatory cytokines such as IL-1β (interleukin-1 beta), IL-6, NO (nitric oxide) and TNF α (tumor necrosis factor alpha). However, the secretion of these cytokines was reduced from the cells treated with the extract of Artemisia Annua L. (EAA). In addition, EAA were able to inhibit inflammatory reaction in the acetaminophen (APAP) -stimulated human hepatocyte carcinoma cell line, HepG2 and to protect hepatocytes from damage caused by various toxins. Acute liver failure animal model was set up with injection of certain concentrations of LPS and D-galN (D-galactosamine) in C57BL/6J mice intraperitoneally, showing highly increased level of AST (aspartate transaminase) and ALT (alanine transaminase). Oral administration of EAA (100 mg/Kg) for 2 weeks reduced the level of AST and ALT up to 50% level of the negative control group treated with water vehicle. The efficacy of EAA was even more effective than the one of the comparative positive control group treated with the extract of Milk thistle. Moreover, EAA protected hepatic cells and tissues from oxidative stresses and inflammatory damages. Taken together, Artemisia Annua L. demonstrated the possibilities as a promising nutraceutical candidate for protection and recovery of liver damages as well as maintain of liver health.