Hyunbeom Lee
Korea Institute of Science and Technology, Republic of Korea
Title: The antiadipogenic effect of Aster glehni extract through HMG-CoA reductase and fatty acid synthase inhibition
Biography
Biography: Hyunbeom Lee
Abstract
Aster glehni Franceht et Sckmidt (AG), a natural product known to have anti-adipogenic, anti-inflammatory, and anticonvulsant effects. To understand the underlying mechanistic role of the natural product for its antiadipogenic effects, the active fractions of the AG extract were tested for their inhibitory effects on HMG-CoA reductase (HMGR) and fatty acid synthase (FAS), two of the most important enzymes involved in the cholesterol and fatty acid synthesis, respectively. We found that dicaffeoylquinic acid methyl esters present in AG are responsible for the inhibition of HMGR and thus we have demonstrated that the overall inhibitory effects of the AG extract against HMGR can be improved by simple methylation of the extract. Subsequent cell-based study found that AGEM70M also have an effect of lowering the rate of cancer cell proliferation.
In addition, global metabolic profiling of an in vivo model using UPLC-Q-TOF-MS after consuming the natural product, we have observed various changes in lipid metabolites such as lysophosphatidylcholine, phosphatidylcholine, and amino acids that are involved in energy metabolism. We believe the natural product AG holds promising therapeutic potentials for not only for cholesterol- and lipid-lowering effects through HMGR and FAS inhibition, but also for anti-proliferative activities against cancer cells.
