P. Sailaja Rao
Sri Venkateshwara College of Pharmacy, India
Title: Preparation and Evaluation of Ethosomal gel of Mangifera indica for wound healing activity in rats
Biography
Biography: P. Sailaja Rao
Abstract
The objective of the present research work is to develop and characterize an ethosomal gel from methanolic leaf extract of Mangifera indica and evaluating the same for wound healing activity. There are many reports revealing the pharmacological activity of the herbal extract but only few were reported as suitable dosage form. Different formulations of three doses (100 mg, 200 mg, 300 mg) of ethosomes using lecithin, cholesterol and ethanol were prepared from Mangifera indica herbal leaf extract. The entrapment efficiency of ethosomes was 65.1% - 96.54% and average vesicle size was 926 nm. Based on the entrapment efficiency and drug release further incorporation into gel formulations using carbopol 940 was done. Wound healing activity was evaluated by excision wound healing model for the three formulations using rats as experimental animals. Animals are divided into 5 groups (n=6) and silver nitrate was used as standard. The % wound area, wound area and period of epithelization were determined. The pH of the gel formulations was found to be in the range of 5.4 - 6.2 with drug content of 50.47%-83.53%. In the wound healing activity, the wound healing effect was studies for a period of 21 days and the treated (200 mg) animals showed significant reduction in % wound area, wound area and period of epithelization. The order of wound healing efficacy of test formulations was found to be 200 mg/kg >100mg/kg >300 mg/kg >standard.
The present study revealed that ethosomal gel applied topically possess wound healing activity.
