Selvaraj Kunjiappan
Kalasalingam University, India
Title: Design, in silico modeling, biodistribution study of rutin and quercetin loaded-stable human hair keratin nanoparticles intended for anticancer targeted drug delivery
Biography
Biography: Selvaraj Kunjiappan
Abstract
The drugs with enhanced effectiveness and least side effects is a major task in current drug discovery. To achieve this immense target, the human hair keratin and rutin-quercetin (Ru-Qr) were chosen to formulate nanoparticles (NPs). The specific drug delivery is a core path to produce significant biological activity, in this connection, the current study was designed to produce high stable Ru-Qr NPs and their characterization such as encapsulation of Ru-Qr, nature, molecular shape, particle size, stability and polydispersity index by FTIR, XRD, SEM, TEM and Zetasize analyzer. The prepared NPs were around ~51 nm size, majorly spherical in shape, crystalline nature and average particle size of 123±5.6 nm, zeta potential to be -28±2.7 mV and polydispersity index of 0.52±0.02. Based on review report, the drug targets 521P and 5P21 were chosen to perform in silico study. Interestingly, the observed in silico study reports shows the strong interaction of NPs and binding pockets of H-Ras P21 protooncogene. In this view, the attracted attention of NPs encouraged and prompted us to study the biodistribution and in vitro anticancer activity by cancer cell line. The initiated research effort towards biodistribution investigation of NPs displayed that, it was penetrated after 3 days of injection, up to 14 % in liver, 18 % in kidney, 8 % in spleen, 3 % in heart and 0% in brain. At 50 µg/mL concentration NPs displayed 78.02% viability in normal liver cell line and 95.60% cytotoxicity in HeLa cell line. The obtained results showed the active NPs enhancing controlled, site specific drug delivery and it can serve as a novel nanodrug in the management of cancer.
Keywords: Keratin, Protooncogene, Multidrug resistance, H-Ras P21, Docking