Iraj Sharifi
Kerman University of Medical Sciences, Iran
Title: Antileishmanial , cytotoxicity and metacaspase gene activities of green tea extract and Glucantime alone or in combination against Leishmania tropica stages and their apoptotic and necrotic effects
Biography
Biography: Iraj Sharifi
Abstract
Leishmaniasis remains a serious global health challenge with no effective therapeutic drug. This study was aimed to evaluate the antileishmanial, cytotoxicity and metacaspase gene expression activities of green tea extract (Epigallocatechin 3-0-Gallate (EGCG) and Glucantime alone or in combination on L.tropica promastigotes and amastigotes by colorimetric assay and macrophage model. Moreover, the apoptotic and necrotic values were assessed by flow cytometry analysis. EGCG was prepared by maceration and various concentratons of EGCG or Glucantime (10, 25, 50, 75 and 100 mg/ml) was prepared. After standard time of 72h treatment of promastigotes, optical density was measured by ELISA to determine the IC50 value (50% inhibitory concentration). For clinical stage, intramacrophage-amastigotes were incubated at various concentrations of EGCG alone or in combination with Glucantime (at minimum inhibitory concentration). The overall antileishmanial potency of EGCG coupled with Glucantime was greater than EGCG or Glucantime alone. No cytotoxicity was induced by EGCG, metacaspase gene was expressed and the extract induced a good index of apoptosis, a possible mechanism of parasite death. Further investigation are essential to elucidate the exact mechanism of action of this potent antioxidant in the chemistry of oxidative stress and its biological activities in clinical setting.
