Harshata Pal
Amity Institute of Biotechnology, India
Title: Molecular Authentication of Endangered Medicinal Plant Panax assamicus and Panax bipinnatifidus from North-East India by Species Specific AFLP Marker
Biography
Biography: Harshata Pal
Abstract
Panax assamicus R. N. Banerjee and Panax bipinnatifidus Seem are two most important endangered species rarely found in North-East India. Due to overexploitation this medicinally valuable plant, it is under the threat of extinction in North-East India. Therefore, molecular documentation of few accessions of Panax assamicus and Panax bipinnatifidus from different locations in Meghalay and Arunachal Pradesh was carried out to develop species specific Amplified Fragment Length Polymorphism (AFLP) marker in automated DNA sequencer. Unique set of common alleles generated from electropherogram in AFLP analysis, depending on primer pairs viz. EcoRIACA*/MSeI-CTA, EcoRIAAG*/MSeICTC, EcoRIACG*/ MSeI-CTG and EcoRI-AGC*/MSeI-CAA, for different accessions of same species are regarded as species specific molecular markers. Both the species Panax assamicus and Panax bipinnatifidus showed 85.25% and 94.55% polymorphism, respectively. The similarity coefficient matrix reflected genetic relatedness among Panax landraces of two species. Cluster I is comprised of four land races of Panax bipinnatifidus and Cluster II is consisted four land races of Panax assamicus in the dendogram, depicting the significance of molecular taxonomy over classical taxonomy to resolute taxonomic complexity of Panax species in NE India. Along with the information on molecular authentication to resist adulteration of these two endangered species the recent research also provides species specific DNA marker relating to catalogue these plant genetic resources